Publication

Methylglyoxal is a byproduct of glycolysis, which readily reacts with proteins to produce advanced glycated endproducts (AGEs). This excessive accumulation has been reported to be associated with the development of various diseases. On the other hand, creatine responsible for energy metabolism in vivo. However, its effect on AGEs accumulation in the brain has not been examined. This study highlighted the strong reactivity of creatine with methylglyoxal. The structure of those reaction products was also estimated by LC-MS/MS analysis. Furthermore, examination using SH-SY5Y cells revealed that creatine inhibits methylglyoxal-induced cytotoxicity and accumulation of AGEs. Future research is anticipated to explore the therapeutic effects of creatine on neurodegenerative diseases.